Classic Broken Cell and Newer Live Cell Methods for Cell Cycle Assessment.

TitleClassic Broken Cell and Newer Live Cell Methods for Cell Cycle Assessment.
Publication TypeJournal Article
Year of Publication2013
AuthorsHenderson, L, Bortone, DS, Lim, C, Zambon, AC
JournalAm J Physiol Cell Physiol
Date Published2013 Feb 7
ISSN1522-1563
ISBN NumberGenomics
iDASH CategoryCell Biology
AbstractMany common, important diseases are either caused or exacerbated by hyperactivation (e.g. cancer) or inactivation (e.g. heart failure) of the cell division cycle. A better understanding of the cell cycle is critical for interpreting numerous types of physiological changes in cells. Moreover, insights into how to control it will facilitate new therapeutics for a variety of diseases and new avenues in regenerative medicine. The progression of cells through the four main phases of their division cycle (G(0)/G(1), S [DNA synthesis], G(2) and M [mitosis]) is a highly conserved process orchestrated by several pathways (e.g. transcription, phosphorylation, nuclear import/export and protein ubiquitination) that coordinate a core cell cycle pathway. This core pathway can also receive inputs that are cell-type and cell-niche dependent. "Broken cell" methods (e.g. use of labeled nucleotide analogues) to assess for cell cycle activity have revealed important insights regarding the cell cycle but lack the ability to assess living cells in real time (longitudinal studies) and with single cell resolution. Moreover, such methods often require cell synchronization, which can perturb the pathway under study. Live-cell-cycle sensors can be used at single-cell resolution in living cells, intact tissue, and in whole animals. Use of these more recently available sensors have the potential to reveal physiologically relevant insights regarding the normal and perturbed cell division cycle.
DOI10.1152/ajpcell.00006.2013
Alternate JournalAm. J. Physiol., Cell Physiol.
PubMed ID23392113

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